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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Nature. 2017 Dec 13;552(7685):410–414. doi: 10.1038/nature25146

Figure 1 |. Cellular localization of phosphoinositide-specific, auxilinl-based PtdIns(4,5)P2 sensors.

Figure 1 |

a, Left, domain organization of mammalian Auxl and of fluorescently tagged Auxl-based phosphoinositide sensors. Right, diagram of sensor-coat association. b, Localization of a general PtdIns(4,5)P2 sensor (EGFP-PH(PLCδl)), a mutated Auxl-based PtdIns(4,5)P2 sensor defective in binding PtdIns(4,5)P2 (EGFP-PH(PLCδl)-mt-Auxl), and a wild-type Auxl-based PtdIns(4,5)P2 sensor (EGFP-PH(PLCδl)-Auxl). Top, distribution of PtdIns(4,5)P2 sensor at a single time point; middle, CLTA-TagRFP superposed on PtdIns(4,5)P2 sensor (green), including enlarged region (square box); bottom, corresponding kymographs from 300-s time series imaged every 2 s by spinning-disk confocal microscopy. EGFP channel in the enlarged regions and kymographs shifted laterally by six pixels. Images are representative of at least three independent experiments. Scale bars, 5 μm.