Extended data Figure 8. nkx1-1 specifies circular muscle fibers.

a, A minor fraction of nkx1-1+ cells co-express the neoblast marker smedwi-1 and b, expression of nkx1-1 within the collagen+ BWM in an uninjured animal (5 animals, 2 experiments). c, Animals become wider after nkx1-1 RNAi. (quantification is in Fig. 3b, 13 control and 17 nkx1-1(RNAi) animals, 3 experiments). d, Loss of circular fibers in nkx1-1(RNAi) animals (quantification in Fig. 3c). Scale bars zoom-ins, 10 μm. d, Comparable numbers of diagonal fibers in nkx1-1 and control RNAi animals (3 experiments). Scale bars, 10 μm. e, Heatmap shows 43 muscle-enriched genes downregulated (log₂ fold-change <0, padj-value<0.001) in uninjured myoD RNAi and nkx1-1 RNAi animals. Each column represents a replicate. f, Co-expression of nkx1-1 and PCGs (top) and reduced PCG expression in uninjured nkx1-1(RNAi) animals after 12 RNAi feedings. Numbers in white indicate double positive cells within the total number of counted cells expressing the PCG (green). (3 experiments). Red box on cartoons depicts location of image shown. Bottom left number: animals with phenotype out of total tested. Anterior, up. Scale bars, 100 μm unless indicated.