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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Nature. 2017 Nov 22;551(7682):623–628. doi: 10.1038/nature24660

Extended data Figure 4. myoD is required for regeneration.

Extended data Figure 4.

a, Normal wound contraction in myoD(RNAi) animals, trunk fragments are shown 30 minutes after amputation (15 animals, 3 experiments). b, Lack of anterior (notum+) and posterior (wnt1+) pole cells (top) and BWM structure (bottom) at 72hpa during regeneration in myoD(RNAi) animals, or c, in an uninjured animals (10 animals/group, 3 experiments). Scale bars, 50 μm. d, Neoblasts did not specify into eye progenitors (ovo+) in myoD(RNAi) animals at 72hpa (1 experiment). e, Homeostatic eye replacement at 11 days following eye resection in myoD(RNAi) animals (10 animals/group, 1 experiment). Scale bars, 500 μm. f, Normal epidermis, neoblast and muscle expression of wound-induced genes in myoD(RNAi) animals 6hpa. g, Graphs show reduced numbers of notum+ and follistatin+ cells in myoD(RNAi) animals at 18hpa, and longitudinal fibers after different numbers of RNAi feedings. Cartoon shows the region counted. Linear correlation between follistatin+ cells and longitudinal fibers. Regression coeficient, R2= 0.6928. Two-tailed Student-t test was performed. p-values are shown in graphs. Mean ±SD are shown in all graphs. Bottom left number: animals with phenotype out of total tested. Anterior, up. Scale bars, 100 μm unless indicated.