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. 2018 Nov 22;25(1):1811–1825. doi: 10.1080/10717544.2018.1494224

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Induction of cell apoptosis by F/A-PLGA@DOX/SPIO NPs. (a) Proliferative inhibition of F/A-PLGA@DOX/SPIO against A549 and L02 cells for 72 h. A549 cells (b) and L02 cells (c) were treated with different concentrations of DOX, FA-PLGA@DOX/SPIO, ACPP-PLGA@DOX/SPIO, and F/A-PLGA@DOX/SPIO for 72 h. The cell viabilities were examined by MTT assay. (d) Flow cytometric analysis of A549 cell cycle distribution. (e) Overproduction of ROS in A549 cells detected by DHE fluorescence intensity. (f) Changes of ROS level in A549 cells incubated with DOX or F/A-PLGA@DOX/SPIO NPs for different periods of time. Values expressed as means ± SD of triplicate. *p < .05 vs. control. **p < .01 vs. control.