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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Neuropharmacology. 2017 Oct 25;128:301–313. doi: 10.1016/j.neuropharm.2017.10.026

Fig. 3. Functional partnership between native group-I and group-II mGlu receptors is mediated by mGlu3 and mGlu5.

Fig. 3.

Stimulation of PI hydrolysis by DHPG and/or LY379268 in cortical slices prepared from wild-type, mGlu2−/− and mGlu3−/− mice are shown in (A–C), where wild-type data at PND9/10 and 60 are the same data as in Fig. 2B for comparative purposes. Data with LY379268 at PND9/10 are highlighted in (A′). The effect of the mGlu3 receptor NAM, VU0650786, on DHPG/LY379268-stimulated PI hydrolysis is shown in (D–F). The effect of the mGlu2 receptor NAM, VU6001966, on DHPG/LY379268-stimulated PI hydrolysis is shown in (G). The effect of the mGlu1 receptor NAM, JNJ16259685, and the mGlu5 receptor NAM, MPEP, on DHPG/LY379268-stimulated PI hydrolysis is shown in (H), where control data are the same data as in Fig. 2B at PND13/14 for comparative purposes. Stimulation of PI hydrolysis by DHPG/LY379268 in cortical slices from mGlu5−/− or crv4 mice and their wild-type counterparts is shown in (I) and (J), respectively. Values are mean ± S.E.M. of 3–5 determinations. p < 0.05 (Two-way ANOVA + Bonferroni test) * vs. the respective basal values and # vs. the DHPG alone or DHPG + LY379268 values obtained in wild-type and mGlu2−/− mice (A: F(2,33) = 110.73 for genotype factor and F(3,33) = 693.82 for treatment factor; A’: F(2,15) = 36.15 for genotype factor and F(3,15) = 77.46 for treatment factor; B: F(2,30) = 36.33 for genotype factor and F(3,30) = 235.35 for treatment factor; C: F(2,35) = 24.79 for genotype factor and F(2,35) = 40.05 for treatment factor). p < 0.05 (Two-way ANOVA + Bonferroni test) * vs. the respective basal values and # vs. the DHPG values (D: F(1,26) = 913.61 for Basal/DHPG factor and F(4,26) = 13.95 for VU0650786 concentrations factor; E: F(1,14) = 70.67 for Basal/DHPG factor and F(3,14) = 57.33 for drugs factor; F: F(3,31) = 29.60 for drugs factor and F(2,31) = 8.56 for VU0650786 concentrations factor; G: F(3,28) = 234.24 for drugs factor and F(1,28) = 9.89 for VU6001966 concentrations factor; H: F(2,24) = 93.54 for drugs - JNJ16259685 and MPEP - factor and F(3,24) = 284.48 for treatment factor. p < 0.05 (Two-way ANOVA + Bonferroni test) * vs. the respective basal values and # vs. the respective DHPG alone values (I: F(1,32) = 2826.74 for genotype factor and F(3,32) = 1763.58 for treatment factor; J: F(1,22) = 160.79 for genotype factor and F(3,22) = 236.83 for treatment factor).