(A) Application of the mGlu2/3 receptor agonist LY379268 (100 nM) for 3 min does not generate a long-term change in the amplitude of the excitatory postsynaptic current (EPSC) (102 ± 14% baseline, n = 4). (B) Application of the mGlu1/5 receptor agonist DHPG (100 μM) for 3 min does not alter long-term excitatory transmission (108 ± 15% baseline, n = 4). (C) Representative experiment showing LTD induced by the combined bath application of LY379268 and DHPG. Inset, representative EPSC traces before and after LTD. Scale bars denote 50 ms and 50 pA. (D) Summary time course of LTD experiments (65 ± 4% baseline, n = 5). (E) VU0650786 blocked the induction of LTD (94 ± 11% baseline, n = 4). (F) MTEP blocked the induction of LTD (100 ± 12% baseline, n = 4). Values are mean ± S.E.M. in A-F.