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. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: Curr Top Membr. 2018 Oct 15;82:141–195. doi: 10.1016/bs.ctm.2018.09.003

Figure 3. Combined super-resolution and atomic force microscopy.

Figure 3.

(A) Components of a typical atomic force microscope (AFM) are depicted. AFM employs an exquisitely sensitive cantilever upon which a laser beam is reflected and a photodetector senses laser beam deflections to map the cell surface at atomic resolution of 0.1 nm in both lateral and axial dimensions. Adapted from Shan and Wang, 2015. (B) Representative image of a live murine astrocyte obtained by sequential, correlated STED-AFM microscopy. Super-resolution STED images (top inset) reveal polarized F-actin in the lamella and near the leading edge. Actin labelled with SiR-actin can be seen with corresponding thick filaments in AFM images (bottom panel) associated with focal adhesions (arrows). Reprinted with permission from Curry, Ghezali et al. 2017.