FIGURE 2. Neutrophils, basophils, and monocytes exhibit distinct kinetics in blood during homeostasis.

Single-bolus BrdU (60 mg/kg body weight) was administrated intravenously to eleven rhesus macaques aged 5 – 10 years old and EDTA-treated blood samples were collected 1, 2, 4, 7, and 14 days later for staining and flow cytometry analysis. Neutrophils (A), basophils (B), and classical monocytes (C) were gated based on the strategy described in Figure 1A and the percentages of BrdU-labeled cells were measured in each subset. Results from monocytes in panel C were previously published by Sugimoto et al. (8) and presented here for comparison with permission of the publisher.