Skip to main content
. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: J Immunol. 2018 May 4;200(12):4059–4067. doi: 10.4049/jimmunol.1800207

FIGURE 4. Gating strategy and BrdU kinetics of neutrophil- and basophil-committed progenitor cells in bone marrow.

FIGURE 4

Bone marrow aspirates were collected, stained and analyzed 1, 4, 7, and 10 days after intravenous BrdU administration from 8 of the 11 animals assessed in Fig. 2. (A) Neutrophil-differentiating cells were gated as Singlet/CD45+/FSC-SSChigh/CD3−/CD20−/CD11b+/HLA-DR−. (C) Basophil-differentiating cells were gated as singlet/FSC-SSChigh/CD3−/CD20−/CD123bright/HLA-DR−. The percent of BrdU-labeled (B) neutrophil- and (D) basophil-committed cells in bone marrow was low at day 1, increased at day 4, and then was declining by days 7 - 10.