Table 1.
The design and results of optimizing immobilization conditions of β-glucosidase by orthogonal experiment. (A) Concentration of sodium alginate (w/v); (B) Concentration of glutaraldehyde (v/v); (C) Concentration of CaCl2 (w/v).
| Entry | Factor | Immobilization efficiency (%) | ||
|---|---|---|---|---|
| A (%) | B (%) | C (%) | ||
| 1 | 1 | 0.1 | 0.5 | 30.12 |
| 2 | 1 | 0.5 | 1 | 30.6 |
| 3 | 1 | 0.8 | 1.5 | 36.44 |
| 4 | 2 | 0.1 | 1 | 30.68 |
| 5 | 2 | 0.5 | 1.5 | 25.92 |
| 6 | 2 | 0.8 | 0.5 | 35.48 |
| 7 | 3 | 0.1 | 1.5 | 31.24 |
| 8 | 3 | 0.5 | 0.5 | 35.88 |
| 9 | 3 | 0.8 | 1 | 38.32 |
| K1 | 32.39 | 30.68 | 33.83 | |
| K2 | 30.69 | 30.8 | 33.2 | |
| K3 | 35.15 | 36.75 | 31.2 | |
| R | 4.46 | 6.07 | 2.63 | |