Fig. 5.

miR-96 inhibitor results in a decrease in the expression of iNOS in dopaminergic neuronal tissues. a iNOS immunohistochemistry for dopaminergic neuronal tissues among seven groups; b iNOS positive cells among seven groups under a microscope; c immunohistochemistry of colocalization analysis between the TH and iNOS; d mRNA expression of iNOS among seven groups evaluated by RT-qPCR; e protein expression of iNOS among seven groups; f protein bands of iNOS among seven groups evaluated by Western blot analysis; ^*, P < 0.05 compared with the normal group, ^#, P < 0.05 compared with the PD and NC groups; Bar = 50 μm. The test was repeated three times independently, and the one-way ANOVA analysis was used for comparison among multiple groups (n = 10 in each group). iNOS, inducible nitric oxide synthase; RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-96, microRNA-96; CACNG5, calcium voltage-gated channel auxiliary subunit gamma 5; PD, Parkinson’ disease; NC, negative control