Figure 2. Distribution of neurons that express hM3D-mCherry in the spinal dorsal horn of VGluT2-Cre mice.
A. Schematic representation of double-floxed Cre-dependent AAV8 vector expressing hM3D and mCherry downstream of the human synapsin promoter (hSyn). ITR, inverted terminal repeat. B and C. Low- and high-magnification confocal images show the distribution of mCherry-labeled (red) and NeuN-immunoreactive (green) neurons in the spinal dorsal horn of VGluT2-Cre mice 2 weeks after microinjection of the AAV-h3MD-mCherry vector. White dashed lines indicate the border of lamina II.
