1A. Female mice were treated with HF diet (60% fat) for 12 weeks at the age of week 4 (experimental Week 0). Body weight of each group were recorded at week 1 and week 12. At experimental week 12, the mice either continued this diet throughout gestation and lactation (the HF group) or were switched to a NF diet 1 week (the H1N group), 5 weeks (the H5N group) or 9 weeks (the H9N group) before conception and continued this diet throughout gestation and lactation. The body weight of HF, H1N, H5N and H9N groups were recorded before pregnancy (T1), after delivery (T2) and after lactation period (T3).
1B and C. Mean body weight of the mothers before pregnancy, after delivery and after lactation. The results are presented as Mean ± SE, n=3-7.
1D and E. IPGTT of the mothers before pregnancy and after lactation. The results are presented as Mean ± SE, n=3-7.