Table 2.
Primers used for mRNA quantification by real-time PCR.
| Gene | Forward primer 5’−3’ | Reverse primer 5’−3’ |
|---|---|---|
| Pklr | CACTCAGCTACAGACCCGTG | CCACACTGTCTTTGCATCGC |
| Pfkfb1 | CTCTACGAAGCTCACACGCT | AACTGGCTCAAGACGCTGAA |
| Pfkl | TTTTGGAGGTGATGGGACGG | TCCATGTTGTCTGGGCGAAA |
| G6pc | GCTGGAGTCTTGTCAGGCAT | ATCCAAGCGCGAAACCAAAC |
| Phkal | GGGAACGTGGCGATAAGACA | GCCCACCTTTCACACCAAAC |
| Fbp | GTCGCTCTTTCCACAGGACA | TTGGCATAGCCCTCGTTGAG |
| Pepck | ATGAAAGGCCGCACCATGTA | GCACAGATATGCCCATCCGA |
| Gsk3b | CGAGACACACCTGCACTCTT | TCCGAGCATGTGGAGGGATA |
| Phkγl | TTGACGCCTATGCTTTCCGT | AAGTCCTCCTCCTCAGCCAA |
| Phkb | GATGCTCACAACCGCAACAG | CAAGGCAAACGCAGGGTAAC |
| Phkg1 | TTGACGCCTATGCTTTCCGT | AAGTCCTCCTCCTCAGCCAA |
| Pygl | CCAGCTTGGGCCACTTACTT | GGAGGGACCCAGCTTACTC |
| Srebf1 | AGCAGTCACCAGCTTCAGTC | GGTCATGTTGGAAACCACGC |
| Pparg | TGTAATGGAAGGGCAAAAGG | TGGCTTCCAGTGCATAAGTT |
| Fasn | GGAGGTGGTGATAGCCGGTAT | TGGGTAATCCATAGAGCCCAG |
| Ppargclα | CAAAGCTGAAGCCCTCTTGC | CCTTTCTTGGTGGAGTGGCT |
| Cebpb | TGGACAAGAACAGCAACGAG | GCCATGGCCTTGACCAAGGAG |
| Acaca | AGATGTGCTGGGTCATGTGG | ATCCAGGCCATGTTGAGACG |
| B2m* | TTCTGGTGCTTGTCTCACTGA | CAGTATGTTCGGCTTCCCATTC |
*
B2m was selected as the internal control. Regarding to the criteria for selecting B2m as the internal control please see the supplementary figure 1