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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Curr Protoc Cell Biol. 2018 Sep 25;81(1):e63. doi: 10.1002/cpcb.63

Figure 4.

Figure 4

Assay for in vitro TA insertion into the ER. (A) Scheme of the TA insertion assay. The purified Get3•TA complex was presented into Δget3 rough ER microsomes. Proper insertion of the TA results in the glycosylation of its C-terminal opsin tag in the ER lumen. (B) Top, representative autoradiogram of the TA insertion reaction. Bottom, quantification and analysis of the insertion time course. The line is a fit of the data to Eq 3. Glyc-Bos1 denotes glycosylated Bos1. Adapted from Figures 7B and 7C in (Rao et al., 2016).