Table 1: In vivo Exposure of PAHs.
The nanogram PAH per milliliter of exposure calculations are based on a 20μL dose of 40.0mg/mL SRM1649b PM). The calculations show the ng PAHs/mL of exposure (0.02mL). The 8hr and 24hr air exposures were calculated based on the amount of air a mouse would breath in 8hrs and 24hrs. The amounts were 0.01m3 for 8 hours and 0.03m3 for 24hrs (Raşid et al., 2012). The permissible exposure level of PAHs set for humans by OSHA is 0.2mg/m3 (200,000ng/m3) which is an 8-hr time-weighted average permissible exposure limit (ATSDR, 2008). This table demonstrates that the in vivo exposure the mice receive is within the OSHA standard for humans and can be considered a relevant exposure. Abbreviations: PAH, polycyclic aromatic hydrocarbon(s); SRM, standard reference material; OSHA, Occupational Safety and Health Administration.
| ng PAH per in vivo PM exposure | |||
|---|---|---|---|
| PAHs | ng PAH/exposure | 8hrs | 24hrs |
| Phenanthrene | 3.22 | 322.40 | 107.47 |
| Anthracene | 0.41 | 41.04 | 13.68 |
| Fluoranthene | 4.99 | 499.20 | 166.40 |
| Pyrene | 3.98 | 398.40 | 132.80 |
| Benz[a]anthracene | 1.69 | 168.80 | 56.27 |
| Chrysene | 2.44 | 243.60 | 81.20 |
| Benzo[b]fluoranthene | 4.94 | 494.40 | 164.80 |
| Benzo[k]fluoranthene | 1.36 | 136.16 | 45.39 |
| Benzo[e]pyrene | 2.38 | 237.92 | 79.31 |
| Benzo[a]pyrene | 1.98 | 197.60 | 65.87 |
| Perylene | 0.49 | 49.12 | 16.37 |
| Indeno[1,2,3-cd]pyrene | 2.31 | 231.20 | 77.07 |
| Benzo[g,h,i]perylene | 3.18 | 317.60 | 105.87 |
| Dibenz[a,h]anthracene | 0.24 | 23.52 | 7.84 |
| Picene | 0.32 | 31.92 | 10.64 |
| Sum of PAHs | 33.93 ng/m3 |
3392.88 ng/m3 |
1130.96 ng/m3 |