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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: FEBS Lett. 2018 Oct 24;592(22):3759–3768. doi: 10.1002/1873-3468.13271

Fig. 4.

Fig. 4.

In addition to tRNAUTuX, SerRS V8 and V20 improve tRNASecUX and tRNAUTu1-mediated TAG codon suppression. WL30153 cells expressing tRNAUTu1 and SerRS variants were grown on LB plates with 400 mg/l Cm. The same strain expressing tRNASecUX and SerRS variants was grown on LB plates with 20 mg/l Cm; both tRNAs were under the control of lpp promoter, and the cat gene with TAG at codon position 146 was on the same plasmid. SerRS expression was controlled by the trc promoter on the second plasmid.