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Steady-state kinetic parameters for tRNAUTuX for wild-type SerRS and evolved variants. The tRNAUTuX concentrations were corrected for plateau charging (48 %). Enzyme concentrations were: 650 nM wild-type SerRS, 187 nM SerRS V8, 92 nM SerRS V20, 224 nM SerRS V22.
| enzyme | KM (μM) | kcat (min−1) | kcat / KM (min−1 μM−1) | relative improvement |
|---|---|---|---|---|
| wt | 1.3 | 0.075 | 0.057 | 1 |
| V8 | 2.4 | 1.4 | 0.58 | 10 |
| V20 | 1.2 | 0.56 | 0.48 | 8.4 |
| V22 | 7.2 | 1.5 | 0.21 | 3.6 |
