A. Body Weight. Wild type (wt) and glut3+/− pre-gestational mouse body weights in grams when maintained on high fat diet (HFD) or chow diet (CD), n = 7–10/group/genotype. ANOVA models followed by Fisher’s PLSD post-hoc test: *vs. wtCD, #vs. glut3+/−CD. F value (1.881), *wtHFD vs. wtCD (p=0.04)at 6th week; F value (4.32), *wtHFD vs. wtCD (p=0.004), #wtHFD vs. glut3+/−CD (p=0.009)at 7th week; F value (1.853), #wtHFD vs. glut3+/−CD (p=0.05) at 8th week; F value (2.317), *wtHFD vs. wtCD (p=0.05)at 9th week; F value (2.210), *wtHFD vs. wtCD (p=0.04), #wtHFD vs glut3+/−CD (p=0.04)at 10th week; and F value (4.311), *wtHFD vs. wtCD (p=0.006), and #wtHFD vs. glut3+/−CD (p=0.006)at 11th week. B. Fat and skeletal muscle mass: in wt and glut3+/− pre-gestational female mice (3 month old) maintained on HFD or CD (n=6–7/group/genotype) measured by nuclear magnetic resonance (NMR). F value (23.57), *wtHFD vs. wtCD (p=0.0001), #wtHFD vs. glut3+/−CD (p=0.0001), #glut3+/−HFD vs. glut3+/−CD (p=0.01), and †glut3+/−HFD vs. wtHFD (p=0.0002).