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. 2018 Oct 24;18(11):3607. doi: 10.3390/s18113607

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Photograph of the assembled disc. (B) Assembly of the disc consisting of a PET film cover, a top patterned polymethyl methacrylate (PMMA) layer, a MembR valve layer, a bottom patterned PMMA layer, and two double-side adhesive layers. (C) Design of the diagnostic platform including six reservoirs with different solutions, a fibre-packed channel, an aliquoting structure, and six chambers for RNA extraction and RT-LAMP reaction; six different MembR valves, four transfer chambers and waste chambers. Reproduced with permission from [84].