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Thermal stability of Nb28 and Nb28-AP. Nb28 and Nb28-AP at working concontrations were incubated at serial temperatures (35, 45, 55, 65, 75, 85, 95 °C) for 5 min (A) or at 90 °C for various lengths of time (0, 5, 15, 30, 45, 60, 75 min) (B). After cooling to room temperature, the reserved activity of Nb28 and Nb28-AP was measured by an indirect ELISA. The error bars represent the standard deviation of three independent tests.
