Fig. 2.

IL-32γ effects on DMBA/TPA-induced local inflammation and inflammatory cell infiltration. WT and IL-32γ mice were treated with DMBA/TPA for 25 weeks. a Real-time PCR analysis of different inflammatory mediators, TNF-α, IL-1β, IL-6, IL-4, IL-10, IL-13, CXCL1, CXCL2, S100A8 and S100A9, on mRNA isolated from skin tissue extracts. n = 5. *p < 0.05; **p < 0.01; ***p < 0.001. b Representative immunohistochemistry images showing Ly6G+ (granulocytes), CD11b + (monocytes/phagocytes) and F4/80+ (macrophages) cells in the skin sections of WT and IL-32γ mice. Ly6G, CD11b and F4/80 stainings were quantified by counting the number of positive cells in the field. Scale bar, 10 μm. n = 5. c Real-time PCR analysis of mRNA expression of Ly6G, CD11b and F4/80. n = 6. *p < 0.05; **p < 0.01. d Production of PGE₂ in the skin tissues measured by ELISA and mRNA expression of mPGES-1 measured by real-time PCR. n = 6. *p < 0.05