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. 2018 Nov 28;37:293. doi: 10.1186/s13046-018-0943-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — IL-32γ suppresses skin cancer stemness. a and b Effect of IL-32γ on the expression of CD44 and CD133 in skin tumor by western blotting (a) and immunohistochemical analysis (b). Scale bar, 10 μm. n = 4. c Effects of IL-32γ on skin cancer sphere formation. Control and IL-32γ-overexpressing A431 and SK-Mel-28 cells were subject to sphere assay for 10 days. Representative images (top) of skin cancer spheres are shown. n = 3. *p < 0.05. d Expression of CD44 and CD133 were detected in A431 and SK-Mel-28 skin CSCs by western blotting. e Expression of Sox2 was analyzed by real-time PCR. n = 3. *p < 0.05. f Effects of IL-32γ on skin cancer chemotherapy. Skin cancer cells, A431 and SK-Mel-28, were cultured with 5-FU for 24 h. The cell viability was determined by MTT assay. n = 3. *p < 0.05