Table 1.
Experimental details of studies, mouse strains, cell types, route of administration, dose and number of animals studied
| Study | Mouse strain | Cell type | Route | Dose | Number of animals |
|---|---|---|---|---|---|
| BLI short-term biodistribution (and MSOT for RAW macrophages) | BALB/c | mMSC | IV and IC | 1.0 × 106 | Minimum n = 3 for each cell type and administration route |
| BALB/c | mKSC | IV and IC | 1.0 × 106 | ||
| BALB/c | hUC-MSC | IV and IC | 1.0 × 106 | ||
| BALB/c | hBM-MSC | IV and IC | 5.0 × 105 | ||
| BALB/c | hKC | IV and IC | 0.3 × 105 | ||
| BALB/c | Macrophages | IV and IC | 1.0 × 107 | ||
| BLI long-term biodistribution | BALB/c SCID | mMSC | IV | 1.0 × 106 | n = 3 |
| BALB/c SCID | mMSC | IC | 1.0 × 106 | n = 5 | |
| BALB/c | mMSC | IC | 1.0 × 106 | n = 4 | |
| FVB | mMSC | IC | 1.0 × 106 | n = 4 | |
| MF1 | mMSC | IC | 1.0 × 106 | n = 4 | |
| BALB/c SCID | hUC-MSC | IV | 1.0 × 106 | n = 13 | |
| BALB/c SCID | hUC-MSC | IC | 1.0 × 106 | n = 3 | |
| BALB/c SCID | hUC-MSC | IV | 5.0 × 105 | n = 6 | |
| BALB/c SCID | hUC-MSC | IC | 5.0 × 105 | n = 14 | |
| BALB/c SCID | hBM-MSC | IV | 5.0 × 105 | n = 6 | |
| BALB/c SCID | hBM-MSC | IC | 5.0 × 105 | n = 6 | |
| MRI cell tracking | BALB/c | mMSC | IV | 1.0 × 106 | n = 2 |
| BALB/c | mMSC | IC | 1.0 × 106 | n = 7 |
Cell numbers for IC administration had to be individually optimised for each cell type used since mice responded severely to higher numbers of some of the cell types after injection into the left ventricle. Administered cell numbers caused no health problems after IV injection in any of the cell types used