Key resources table.
| Reagent type |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Fly line | R23E07-p65.AD | Bloomington drosophila stock center |
Rrid:BDSC_70131 | Landing site: attP40 |
| Fly line | VT002081-GAL4.DBD | Bloomington Drosophila Stock Center |
RRID: BDSC_73398 | Landing site: attP2 |
| Fly line | UAS-TNTE | (Tastekin and Louis, 2017) | NA | Secondchromosome |
| Fly line | UAS-TNTG | Bloomington Drosophila Stock Center |
RRID: BDSC_28838 | Second chromosome |
| Fly line | 20XUAS-IVS-CsChrimson.mVenus | Bloomington Drosophila Stock Center |
RRID: BDSC_55136 | Landing site: attP2 |
| Fly line | 20XUAS-IVS-CsChrimson.mVenus | Bloomington Drosophila Stock Center |
RRID: BDSC_55134 | Landing site: attP18 |
| Fly line | R57C10-FLP2::PEST | (Nern et al., 2015) | NA | Gift from Aljoscha Nern |
| Fly line | R57C10-FLPL attp8; 10xUAS(FRT.stop)myr ::smGdP-HA VK00005; 10xUAS(Frt.stop)myr::smGdP-V5-THS- 10XUAS(FRT.stop)myr::smGdP-FLAG su(Hw)attP1 |
Bloomington Drosophila Stock Center |
RRID: BDSC_64087 | Gift from Aljoscha Nern |
| Fly line | CG9887-LexA su(Hw)attP8 | NA | NA | Julie Simpson |
| Fly line | 20XUAS(Frt.stop)Chrimson::mVenus attP2 | NA | NA | Gift from Vivek Jayaraman |
| Fly line | 20XUAS-IVS-CsChrimson.mVenus attP18; CG9887-LexA su(Hw)attP8; 12XlexAop2- IVS-GCaMP6f-p10 su(Hw)attP5 |
NA | NA | Gift from Stefan Pulver |
| Fly line | R75C02-GAL4 | (Li et al., 2014) available at Bloomington Drosophila Stock Center |
RRID: BDSC_39886 | Landing site: attP2 |
| Fly line | R75C02-LexA/Cyo | (Li et al., 2014) available at Bloomington Drosophila Stock Center |
RRID: BDSC_54365 | Landing site attP40 |
| Fly line | ChAT RNAi | Vienna Drosophila Resource Center | RRID: FlyBase_FBst0463844 | |
| Fly line | 13xLexAop2-IVS-GCaMP6f-p10 50.693 in VK00005, 20xUAS-CsChrimson-mCherry-trafficked in su(Hw)attp1 | NA | NA | Gift from Vivek Jayaraman |
| Odor | Ethyl butyrate | MilliporeSigma, MO, USA | Cat# 19230 | CAS#: 105-54-4 |
| Odor | Ethyl acetate | MilliporeSigma, MO, USA | Cat# 319902 | CAS#: 141-78-6 |
| Chemical | Paraffin oil | MilliporeSigma, MO, USA | Cat# 18512 | CAS#: 8012-95-1 |
| Chemical | PBS | MilliporeSigma, MO, USA | Cat# P4417 | |
| Chemical | Agarose | Lonza, Basel, Switzerland | Cat# 50001 | |
| Chemical | Bacto Agar | Thermo Fisher Scientific, MA, USA | Cat# DF0812-07-1 | |
| Chemical | Vectashield | Vector Laboratories, CA, USA |
Cat# H1000 | |
| Chemical | Poly-L-lysine solution | MilliporeSigma, MO, USA | Cat# P8920 | CAS#: 25988-63-0 |
| Chemical | Formaldehyde | Thermo Fisher Scientific, MA, USA |
Cat# NC9658705 | |
| Chemical | All trans-retinal | MilliporeSigma, MO, USA | Cat# R2500 | CAS#: 116-31-4 |
| Chemical | Sucrose | MilliporeSigma, MO, USA | Cat# S0389 | CAS#: 57-50-1 |
| Chemical | Sodium Chloride | MilliporeSigma, MO, USA | Cat# S7653 | CAS#: 7647-14-5 |
| Chemical | Calcium Chloride | MilliporeSigma, MO, USA | Cat# 21115 | CAS#: 10043-52-4 |
| Chemical | Magnesium Chloride Hexahydrate | MilliporeSigma, MO, USA | Cat# P2670 | CAS#: 7791-18-6 |
| Chemical | Potassium Chloride | MilliporeSigma, MO, USA | Cat# P9571 | CAS#: 7447-40-7 |
| Chemical | TES | MilliporeSigma, MO, USA | Cat# T1375 | CAS#: 7365-44-8 |
| Chemical | Sylgard 184 | Dow Corning, MI, USA | Cat# 4019862 | |
| Antibody | Mouse anti-brp (nc82) | Development Studies Hybridoma Bank (DSHB), IA, USA |
RRID: AB_2314866 | 1:25 dilution |
| Antibody | Mouse anti-synorf1 (3C11) | Development Studies Hybridoma Bank (DSHB), IA, USA |
RRID: AB_2313867 | 1:25 dilution |
| Antibody | Rabbit anti-GFP | Thermo Fisher Scientific, MA, USA | RRID: AB_221569 | 1:500 dilution |
| Antibody | Mouse anti-GFP | Thermo Fisher Scientific, MA, USA | RRID: AB_221568 | 1:500 dilution |
| Antibody | Mouse anti-ChAT (ChAT4B1) | Development Studies Hybridoma Bank (DSHB), IA, USA |
RRID: AB_528122 | 1:100 dilution |
| Antibody | Rabbit anti-Glutamate | - | RRID: AB_2490070 | 1:1000 dilution Gift from Hermann Aberle |
| Antibody | Rabbit anti-GABA | MilliporeSigma, MO, USA | RRID: AB_472652 | 1:2000 dilution |
| Antibody | Rabbit anti-HA-Tag (C29F4) | Cell Signaling Technology, MA, USA | RRID: AB_1549585 | 1:500 dilution |
| Antibody | Goat anti-Rabbit Alexa Fluor 488 | Thermo Fisher Scientific, MA, USA | RRID: AB_143165 | 1:500 dilution |
| Antibody | Goat anti-Rabbit DyLight 549 | Vector Laboratories, CA, USA | RRID: AB_2336407 | 1:500 dilution |
| Antibody | Goat anti-Mouse Texas Red | Jackson ImmunoResearch Laboratories, PA, USA | Cat# 115-075-003 | Discontinued 1:500 dilution |
| Antibody | Goat anti-Mouse FITC | MilliporeSigma, MO, USA | RRID: AB_259804 | 1:500 dilution |
| Serum | Goat Serum | MilliporeSigma, MO, USA | Cat# G9023 | 3% dilution |
| Consumable | 96 well plate lids with condensation rings |
MilliporeSigma, MO, USA | Cat# L3661 | |
| Consumable | 96 well plate lids without condensation rings |
MilliporeSigma, MO, USA | Cat# L3536 | |
| Consumable | 245 mm x 245 mm Petri dish | MilliporeSigma, MO, USA | Cat# CLS431111 | |
| Software | MATLAB 2015b | Mathworks, MA, USA |
RRID: SCR_001622 | |
| Software | LabView | National Instruments, TX, USA |
RRID: SCR_0014325 | |
| Software | Fiji/ImageJ | Fiji | RRID: SCR_002285 |