Table 1. Resistance genes, class 1 integron gene cassettes and promoter types in SMX/TRI-resistant bacteria from the four wetlands in Ibadan and Lagos, Nigeria.
| Isolates | Phylogenetic placement | Date of isolation | Resistance genes/Integrons | Integron gene cassettes | Promoter Types |
|---|---|---|---|---|---|
| Awba Wetland | |||||
| PAW1-6 | Citrobacter sp. | October 2014 | sul1, sul2, sul3, intI1, intI2 | aadA1|dfrA1 | PcH1-P2 |
| PAW1-4 | Citrobacter sp. | October 2014 | sul1, intI1, intI2 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| PAW2-4 | Enterobacter sp. | November 2014 | sul1, intI1 | aadA2 | PcW-P2 |
| EAW2-1 | Citrobacter sp. | November 2014 | sul1, sul2, intI1 | aadA2 | PcW-P2 |
| EAW2-3 | Enterobacter sp. | November 2014 | sul1, intI1 | aadA2 | PcW-P2 |
| PAW2-6 | Enterobacter sp. | November 2014 | sul1, sul2, intI1 | aadA2 | PcW-P2 |
| PAW3-1 | Enterobacter sp. | December 2014 | sul1, sul2, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| PAW3-3 | Citrobacter sp. | December 2014 | sul1, sul2, intI1, intI2 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| PAW4-5 | Enterobacter sp. | January 2015 | sul1, dfrA7, intI1 | dfrA7 | PcW-P2 |
| EAW4-6 | Citrobacter sp. | January 2015 | sul1, dfrA1, intI1 | aadA1|dfrA1 | PcH1-P2 |
| PAW4-4 | Citrobacter sp | January 2015 | sul1, intI1, intI2 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| Apete Wetland | |||||
| EAP1-2 | Enterobacter sp. | October 2014 | sul1, sul2, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| EAP1-3 | Citrobacter sp. | October 2014 | intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| EAP1-5 | Pseudomonas sp. | October2014 | sul1, sul2, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| PAP2-5 | Citrobacter sp | November 2014 | intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| PAP2-6 | Citrobacter sp. | November 2014 | sul1, sul2, dfrA7, intI1 | dfrA7 | PcW-P2 |
| EAP2-1 | Citrobacter sp. | November 2014 | sul1, dfrA7, intI1 | dfrA7 | PcH1-P2 |
| EAP2-3 | Citrobacter sp. | November 2014 | sul1, sul2, dfrA7, intI1 | dfrA7 | PcW-P2 |
| PAP2-2 | Citrobacter sp. | November 2014 | sul1, sul2, dfrA7, intI1 | dfrA7 | PcW-P2 |
| PAP2-3 | Citrobacter sp. | November 2014 | sul1, sul2, dfrA7, intI1 | dfrA7 | PcW-P2 |
| PAP4-16 | Citrobacter sp. | January 2015 | sul1, dfrA7, intI1 | dfrA7 | PcW-P2 |
| PAP4-3 | Citrobacter sp. | January 2015 | sul1, sul2, dfrA7, intI1 | dfrA7 | PcW-P2 |
| EAP4-6 | Achromobacter sp. | January 2015 | dfrA7, intI1 | dfrA7 | PcH1-P2 |
| EAP1-1 | Citrobacter sp. | January 2015 | sul1, sul2, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| PAP4-5 | Citrobacter sp. | January 2015 | sul1, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| EAP4-1 | Citrobacter sp. | January 2015 | sul1, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcH1-P2 |
| PAP4-1 | Citrobacter sp. | January 2015 | sul1, sul2, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcW-P2 |
| EAP4-5 | Pseudomonas sp. | January 2015 | sul1, sul2, intI1 | acc(6’)lb-cr|arr3| dfrA27 | PcH1-P2 |
| Abule-Agege | |||||
| PAA1-2 | Escherichia sp. | October 2014 | sul1, sul2, intI1 | arr3| acc(6’)lb-cr|dfrA27 | PcW-P2 |
| PAA2-7 | Citrobacter sp. | November 2014 | sul1, intI1 | aadA2 | PcW-P2 |
| PAA2-8 | Citrobacter sp. | November 2014 | sul1, sul2, intI1 | aadA2 | PcW-P2 |
| EAA3-5 | Citrobacter sp. | December 2014 | drfA12, intI1 | arr3| acc(6’)lb-cr|dfrA27 | PcH1-P2 |
| PAA4-1 | Citrobacter sp. | January 2015 | sul1, sul2, intI1 | arr3| acc(6’)lb-cr|dfrA27 | PcW-P2 |
| EAA4-2 | Enterobacter sp. | January 2015 | intI1 | dfrA7 | PcH1-P2 |
ND: Not Detected, PcW: Weak Promoter, PcH1: Hybrid promoter 1, P2: Inactive promoter 2