Fig 3. Multiple nucleoporins and transport receptors are required for anti-viral activity of MX2 in HeLa cells.

(A) HeLa cells were stably transduced with lentiviral vectors constitutively expressing FLAG-tagged MX2 or CD8 (negative control), and transduced cells selected by treatment with 1 μg/ml puromycin for 72 h. After selection, transduced cells were transfected twice, 24 h apart, with 20 nM siRNA targeting individual nucleoporins or transport receptors. A non-targeting siRNA was included as a control, CTRL. After 48 h, cells were challenged with HIV-1/GFP and transduction efficiency assessed by flow cytometry after a further 48 h post challenge. (B) the same data as in A are represented as fold MX2-mediated inhibition, calculated as in Fig 2 (n = 3; mean ± SD; *p-value < 0.05; paired t-test to CTRL siRNA).