Table 1.
Composition | LP1 | LP2 | LP3 | Composition | LP1 | LP2 | LP3 |
---|---|---|---|---|---|---|---|
Neutral polysaccharide | 62.35 ± 2.12 a | 82.45 ± 2.42 b | 90.44 ± 2.12 c | Protein | 33.72 ± 0.16 c | 5.91 ± 0.07 b | 3.20 ± 0.06 a |
Hexuronic acid | 1.98 ± 0.14 a | 9.03 ± 0.40 c | 4.31 ± 0.20 b | Aspartate | 3.90 | 0.73 | 0.31 |
Percentage (%) | Glutamic | 4.34 | 0.96 | 1.00 | |||
Ribose | 3.29 | 0.81 | 1.32 | Serine | 1.74 | 0.25 | 0.12 |
Rhamnose | 2.91 | 0.40 | 0.12 | Glycine | 1.78 | 0.36 | 0.16 |
Arabinose | 28.2 | 6.63 | 3.76 | Threonine | 1.86 | 0.29 | 0.12 |
Xylose | 0.55 | 9.44 | 0.51 | Histidine | 0.86 | 0.10 | 0.05 |
Mannose | 7.20 | 27.3 | 28.9 | Alanine * | 2.58 | 0.62 | 0.41 |
Glucose | 46.8 | 54.6 | 63.9 | Arginine | 1.62 | 0.31 | 0.09 |
Galactose | 11.0 | 0.87 | 1.50 | Tyrosine * | 1.22 | 0.09 | 0.02 |
Molar ratio | Valine * | 2.49 | 0.41 | 0.16 | |||
Ribose | 0.55 | 0.12 | 0.35 | Metione | 0.75 | 0.05 | <0.05 |
Rhamnose | 0.44 | 0.06 | 0.03 | Phenylalanine * | 1.90 | 0.24 | 0.07 |
Arabinose | 4.70 | 1.00 | 1.00 | Isoleucine * | 1.89 | 0.29 | 0.09 |
Xylose | 0.09 | 1.42 | 0.14 | Leucine * | 3.34 | 0.49 | 0.20 |
Mannose | 1.00 | 3.43 | 6.40 | Lysine | 1.98 | 0.46 | 0.16 |
Glucose | 6.50 | 6.86 | 14.16 | Proline * | 1.48 | 0.28 | 0.19 |
Galactose | 1.83 | 0.13 | 0.40 |
The contents of neutral polysaccharide and hexuronic acid were individually determined by the phenol-sulphuric acid method and the method of Blumenkrantz and Asboe-Hansen. The monosaccharide and amino acid compositions of LP1-3 were determined by gas chromatograph/mass spectrometer and reverse phase-high performance liquid chromatography, respectively. The values for neutral polysaccharide, hexuronic acid, and protein were expressed as mean ± SD (n = 3). The results having the different letter in each row are significantly different (P < 0.05), * represents hydrophobic amino acid.