Table 4.
Cellular antioxidant activity of chestnut burs extracts and fractions.
| no PBS wash | PBS wash | cytotoxicity | |||
|---|---|---|---|---|---|
| EC50 (mg/mL) | CAA (μmol of QE/100g) | EC50 (mg/mL) | CAA (μmol of QE/100g) | CC50 (mg/Ml) | |
| water extraction | 38.7 ± 3.5 A | 11.6 ± 0.4 A | 389 ± 34 A | 1.86 ±0.29 A | 249.3 |
| 30% ethanol extraction | 36.5 ± 2.9 A | 11.9 ± 0.6 A | 334 ± 22 B | 1.98 ± 0.26 A | 213.5 |
| 50% ethanol extraction | 33.5 ± 3.1 A | 12.6 ± 0.5 A | 276 ± 19 C | 2.86 ± 0.23 B | 224.9 |
| 75% ethanol extraction | 34.6 ± 3.3 A | 12.3 ± 0.3 A | 266 ± 12 C | 2.71 ± 0.15 B | 306.5 |
| Fraction I | 71.6 ± 4.1 B | 10.2 ± 0.4 B | 556 ± 48 D | 1.11 ± 0.11 C | 300.4 |
| Fraction II | 14.2 ± 2.2 C | 54.2 ± 2.6 C | 109 ± 12 E | 5.66 ±0.20 D | 278.5 |
QE: quercetin equivalent. In each column different letters mean significant differences (p < 0.05).