Table 6.
Immobilization technique | Enzyme | Reaction scheme | Results | Ref. |
---|---|---|---|---|
Covalently immobilized on gold patterned surface | Horseradish peroxidase | Conversion with self-assembled monolayer approach was 1.5 time higher than physical adsorption | [68] | |
Surface modification by sol-gel technique/Ni-NTA immobilization | l-Lactic dehydrogenase | Crude enzyme can be used for immobilization Reversible immobilization was achieved by EDTA treatment Reaction was completed within 15 min |
[47] | |
Entrapment of Novozym-435TM within microchannel | Lipase | Apparent rate of reaction is at least an order higher than that observed for batch reactors | [70] | |
CIM-disk epoxy monolith | Glycosyl-transferases | Immobilized enzyme is stable and exhibits good reproducibility | [37] | |
Entrapment of silica-immobilized enzymes within microchannel | Zinc Hydroxy-aminobenzene mutase Peroxidase |
Used combinatorial synthesis of 2-aminophenoxyazin-3-one | [71] |