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. 2011 Jul 19;16(7):6041–6059. doi: 10.3390/molecules16076041

Table 6.

Oxidation, reduction and miscellaneous reactions in enzyme-immobilized microreactor.

Immobilization technique Enzyme Reaction scheme Results Ref.
Covalently immobilized on gold patterned surface Horseradish peroxidase graphic file with name molecules-16-06041-i013.jpg Conversion with self-assembled monolayer approach was 1.5 time higher than physical adsorption [68]
Surface modification by sol-gel technique/Ni-NTA immobilization l-Lactic dehydrogenase graphic file with name molecules-16-06041-i014.jpg Crude enzyme can be used for immobilization
Reversible immobilization was achieved by EDTA treatment
Reaction was completed within 15 min
[47]
Entrapment of Novozym-435TM within microchannel Lipase graphic file with name molecules-16-06041-i015.jpg Apparent rate of reaction is at least an order higher than that observed for batch reactors [70]
CIM-disk epoxy monolith Glycosyl-transferases graphic file with name molecules-16-06041-i016.jpg Immobilized enzyme is stable and exhibits good reproducibility [37]
Entrapment of silica-immobilized enzymes within microchannel Zinc
Hydroxy-aminobenzene mutase
Peroxidase
graphic file with name molecules-16-06041-i017.jpg Used combinatorial synthesis of 2-aminophenoxyazin-3-one [71]