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. 2011 Nov 14;16(11):9480–9494. doi: 10.3390/molecules16119480

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© 2011 by the authors;

licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) Standard proteins analyzed by size-exclusion HPLC chromatography. Standard markers: bovine serum albumin (BSA, 67 kDa), ovalbumin (OVA, 44 kDa), insulin (Ins, 5.7 kDa) and tryptophan (Trp, 0.2 kDa); (B) AEL by size-exclusion HPLC; (C) Standard curve representing native molecular weight of AEL by size-exclusion HPLC; (D) SDS-PAGE, pH 8.3 purified AEL using 12% gel (running time 3 h at a constant 100 V). Mr, Molecular weight markers (from top to bottom): β-galactosidase (116.0 kDa); bovine serum albumin (66.2 kDa); ovalbumin (45.0 kDa); lactate dehydrogenase (35.0 kDa); REase Bsp981 (25.0 kDa); β-lactoglobulin (18.4 kDa); and lysozyme (14.4 kDa). The gels were stained with silver nitrate.