Table 2.
Summary of IC50, Ki values and inhibition mode of MSE on CYP activities. a Ki values are derived from secondary plots of slopes taken from double reciprocal plots versus MSE concentrations. The compounds were classified as potent (IC50 ≤ 20 µg/mL or ≤10 µM); moderate (IC50 from 20 to 100 µg/mL or 10 to 50 µM) or weak (IC50 from ≥100 µg/mL or ≥50 µM) [14]. Data are the average values of duplicate determinations; b Nd, not determined.
| Cytochrome P450 | IC50 (µg/mL) | Ki (µg/mL) | Mode of inhibition |
|---|---|---|---|
| 3A4/BFC | 0.78 | 1.526 a | Non competitive |
| 2D6/AMMC | 0.636 | 2.6 a | Competitive |
| 1A2/CEC | 39 | 18.57 a | Non competitive |
| 2C19/CEC | Nd b | 84.88 a | Non competitive |