Figure 2.

Effect of CTet formulated in aqueous solutions on DNA synthesis of MCF-7 breast cancer cell line. Cells were treated with various concentrations of CTet suspended in 10% EtOH (Ο), 10% EtOH with 160 mM γ-CD (◊) or pure EtOH (▲); during the last 5 h of treatment, cells were pulsed with [³H]thymidine, and the incorporation into DNA was determined (1.5 μCi). Data are expressed as percentage of cells treated with vehicle only and are means ± SEM of at least three experiments.