Fig 6. Effect of DGAT1 inhibitor on cell-associated and accumulated oleic acid.

(A) Human myotubes were treated with fatty acids (100 μM EPA or PA) for 24 h. Cell-associated [¹⁴C]OA (100 μM) was measured after 4 h with and without DGAT1 inhibitor (D1i, A922500, 1 μM). Results represent mean ± SEM, from experiments with 4 different donors and 4–8 parallels (n = 26). Cell-associated PA (100%): 53.8 ± 4.0 nmol/mg cell protein. (B) Human myotubes were grown and differentiated in 96-well ScintiPlate tissue culture plates. On day 6 of differentiation the myotubes were treated with a mixture of 100 μM fatty acids for 24 h and cell-associated radioactivity was measured during 24 h by SPA. The mixture was trace amounts of [¹⁴C]OA (9 μM) and non-labeled PA or EPA (91 μM). DGAT1 inhibitor was also added for 24 h. Results represent mean ± SEM from experiments with 3 different donors and 8 parallels (n = 24). Accumulated PA (100%): 156 ± 13.8 nmol/mg cell protein. #p<0.05 for D1i inhibitor vs. control in EPA-treated cells (t-test). EPA, eicosapentaenoic acid; PA, palmitic acid; D1i, diacylglycerol acyltransferase 1 inhibitor.