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. 2018 Nov 29;13(11):e0201683. doi: 10.1371/journal.pone.0201683

Fig 3. Demonstration of increased reporter KI efficiency in multiple human PSC lines after transient puromycin treatment.

Fig 3

(A) Representative images of flow cytometry assessment of SOX2-P2A-eGFP KI into EP1 hiPSCs with and without transient puromycin selection followed by flow cytometry analysis. Untransfected cells were used to set the gates for reporter negative cells. n = 2 for both groups. p value = 0.0039. (B) Flow cytometry analysis of TBP-P2A-eGFP KI into EP1 hiPSC, H7 hESC, and IMR90-4 hiPSC lines, respectively, with and without transient puromycin selection. For EP1 and H7, n = 2 for both groups, for IMR90-4 n = 3 for replicates without puromycin and n = 4 for replicates with puromycin treatment. n = biological replicates. p values: EP1 = 0.0246, H7 = 0.1532, IMR90-4 = <0.0001. * = p < .05, ** = p < .01, **** = p < .0001. ns = not significant. Unpaired two tailed t-test was used.