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. 2018 Nov 29;13(11):e0201683. doi: 10.1371/journal.pone.0201683

Fig 5. Dual KI of TBP and MYC or TBP and SOX2 fluorescent reporters into H9 hESCs.

Fig 5

(A) Representative images of flow cytometry assessment of dual KI of TBP-P2A-eGFP and MYC-P2A-tdTomato or TBP-P2A-tdTomato and SOX2-P2A-eGFP into H9 hESCs with and without transient puromycin selection. (B) Flow cytometry analysis of the dual KI described in A. n = 2 for both dual KI combinations. n = biological replicates. (C) Fluorescence and brightfield microscopy of H9 hESCs positive for both reporter genes. Scale bar = 400 μm.