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. 2018 Nov 29;13(11):e0201683. doi: 10.1371/journal.pone.0201683

Fig 7. Zeocin replacement of puromycin results in similar KI efficiency of fluorescent reporter genes into human PSCs.

Fig 7

(A) Representative images of flow cytometry assessment of TBP-P2A-eGFP KI into IMR90-4 hiPSCs and H7 hESCs with and without transient zeocin selection. (B) Flow cytometry analysis of TBP-P2A-eGFP KI in part A. For IMR90-4, n = 3 for replicates without zeocin and n = 4 for replicates with zeocin treatment, for H7 n = 2 for replicates without zeocin and n = 5 for replicates with zeocin treatment. n = biological replicates. p values: IMR90-4 = 0.0172, H7 = 0.0036; * = p < .05, ** = p < .01; Unpaired two tailed t-test was used.