Fig 9. Provision of JNK-IN-8 and TCS JNK 6o in artificial blood meals reduced eggs laid per female A. stephensi during the first gonotrophic cycle.

Three-day old A. stephensi were provided blood meals supplemented with 1 μM JNK-IN-8, TCS JNK 6o, or blood supplemented with an equivalent volume of diluent as a control. At 24 h post feeding, females were housed individually in modified 50 ml conical tubes and provided water for oviposition for two days. Eggs were washed onto filter paper, photographed, and counted using ImageJ software. The experiment was replicated four times with separate cohorts of mosquitoes. (A) Proportions of females that laid at least one egg were analyzed by Chi-square test. (B) Clutch sizes relative to controls were analyzed by Mann-Whitney test (NS = not significant).