Figure 5.
Differential Expression Analysis of KO Lines
(A) Scheme showing mutant embryo collection for RNA-seq at 6–8 hr after egg laying (AEL).
(B) Global differential expression changes: volcano plots show significantly affected genes for XLOC_012319 and XLOC_012225 knockout (KO) embryos, comparing two replicates from the KO against all other samples (including WT parental line and other mutants). Red dots depict genes with adjusted p value < 0.05, and orange dots indicate genes with sub-threshold significance but >2-fold change in mean expression.
(C) Local differential expression: raw p values (y axis) and log2 fold change in expression (color scale) of genes located ±500,000 kbp from the center of the deletions (indicated by vertical gray boxes).