Table 1.
Viability Assessment of Knockout Lines
| KO Line | Temperature (°C) | Homozygous Progeny | Heterozygous Progeny | Het/Hom Ratio |
|---|---|---|---|---|
| XLOC_004366 (a) | 25 | 22 | 47 | 2.1 |
| XLOC_004366 (a) | 29 | 16 | 34 | 2.1 |
| XLOC_004366 (b) | 25 | 30 | 61 | 2.0 |
| XLOC_004366 (b) | 29 | 25 | 45 | 1.8 |
| XLOC_012225 (a) | 25 | 22 | 40 | 1.8 |
| XLOC_012225 (a) | 29 | 17 | 36 | 2.1 |
| XLOC_012225 (b) | 25 | 36 | 65 | 1.8 |
| XLOC_012225 (b) | 29 | 23 | 50 | 2.2 |
| XLOC_012319 | 25 | 22 | 42 | 1.9 |
| XLOC_012319 | 29 | 16 | 34 | 2.1 |
Siblings heterozygous KO stocks (deletion over balancer) were crossed at normal (25°C) or restrictive (29°C) temperatures, and the genotype of the progeny was evaluated using visible markers from the balancer chromosome. The expected proportions of adults if the KO is viable is 2/3 heterozygous (KO over balancer) and 1/3 homozygous KO, or a 2:1 Het/Hom ratio (homozygous balancer chromosomes are embryonic lethal).