Table 2.
Tm values from Bicolor FMCA of the PRV strains used in this study
| Name | GenBank accession no. | Tm value (°C) | ΔTm value (°C)a | Genotype | Interpretation | ||
|---|---|---|---|---|---|---|---|
| P1 (FAM channel) | P2 (HEX channel) | P1 (FAM channel) | P2 (HEX channel) | ||||
| p-B | — | 70.53 | 71.61 | 0 | 0 | Genotype I (Bartha-K61) | reference control/plasmid |
| p-N | — | 70.53 | 75.99 | 0 | 4.38 | Genotype I | positive control/plasmid |
| p-H | — | 76.88 | 75.98 | 6.35 | 4.37 | Genotype II | positive control/plasmid |
| Bartha-K61 | JF797217.1 | 70.50 | 71.59 | -0.03 | -0.02 | Genotype I (Bartha-K61) | positive sample |
| NIA3 | KU900059.1 | 70.28 | 75.96 | -0.25 | 4.35 | Genotype I | positive sample |
| HDDJ | MF434048 | 76.70 | 76.00 | 6.17 | 4.39 | Genotype II | positive sample/field sample |
| GDGZ | MF434045 | 76.75 | 75.84 | 6.22 | 4.23 | Genotype II | field sample |
| GDZQ | MF434042 | 76.93 | 76.20 | 6.4 | 4.59 | Genotype II | field sample |
| GDHY | MF434044 | 77.13 | 76.21 | 6.6 | 4.6 | Genotype II | field sample |
| GDHY2 | MF434038 | 76.70 | 76.00 | 6.17 | 4.39 | Genotype II | field sample |
| GDZQ2 | MF434039 | 76.95 | 76.04 | 6.42 | 4.43 | Genotype II | field sample |
| GDSD | MF434043 | 76.74 | 76.04 | 6.21 | 4.43 | Genotype II | field sample |
| JXGZ | MF434047 | 76.67 | 75.97 | 6.14 | 4.36 | Genotype II | field sample |
| JXGZ2 | MF434036 | 76.63 | 75.93 | 6.1 | 4.32 | Genotype II | field sample |
| LC | MF434047 | 76.69 | 75.98 | 6.16 | 4.37 | Genotype II | field sample |
| HNCS | MF434046 | 76.51 | 75.81 | 5.98 | 4.20 | Genotype II | field sample |
| GDHY8 | MH053134 | 77.27 | 76.16 | 6.74 | 4.55 | Genotype II | field sample |
| GDGZ2 | MH053135 | 76.87 | 76.15 | 6.34 | 4.54 | Genotype II | field sample |
| GDBL | MH053136 | 76.75 | 76.03 | 6.22 | 4.42 | Genotype II | field sample |
| SXDT | MH053137 | 76.14 | 76.24 | 5.61 | 4.63 | Genotype II | field sample |
| HNCS4 | MH053138 | 76.99 | 76.08 | 6.46 | 4.47 | Genotype II | field sample |
| GDHZ | MH053139 | 76.91 | 76.00 | 6.38 | 4.39 | Genotype II | field sample |
| GDQY | MH053140 | 76.92 | 76.01 | 6.39 | 4.40 | Genotype II | field sample |
| GDHY5 | —b | 70.49 | 71.60 | -0.04 | -0.01 | Genotype I (Bartha-K61) | field sample |
| GDZQ4 | —b | 70.51 | 71.61 | -0.02 | 0 | Genotype I (Bartha-K61) | field sample |
| JXGZ4 | —b | 70.57 | 71.66 | 0.03 | 0.05 | Genotype I (Bartha-K61) | field sample |
| GDSG | —b | 70.56 | 71.61 | 0.03 | 0 | Genotype I (Bartha-K61) | field sample |
| GDMM | —b | 70.45 | 71.56 | -0.08 | -0.05 | Genotype I (Bartha-K61) | field sample |
| GDFS | —b | 70.71 | 71.61 | 0.18 | 0 | Genotype I (Bartha-K61) | field sample |
| GDZS | —b | 70.48 | 71.57 | -0.05 | -0.04 | Genotype I (Bartha-K61) | field sample |
| GDQY3 | —b | 70.55 | 71.64 | 0.02 | 0.03 | Genotype I (Bartha-K61) | field sample |
aΔTm = Tm value of detected samples minus Tm of reference plasmid p-B in each channel. bThe detected strains shared 100% identities in gC nucleotide sequence to Bartha-K61 strain (JF797217.1).