Fig. 4.

miR-6868-5p inhibits CRC angiogenesis by targeting FOXM1. a HUVECs were treated with the CM from HCT116 cells transfected with indicated vectors. Cell viability of HUVECs was measured by CCK8 assay. b HUVECs were co-cultured with HCT116 cells transfected with indicated vectors in transwell apparatus. Migrated HUVECs were quantified after co-culture for 24 h. Scale bar = 20 μm. c HUVECs were treated with the CM from indicated cells, and subjected to tube formation assay. d Representative images of IHC staining for CD31 in control and FOXM1 overexpressing tumors. Scale bar = 20 μm. e Western blot analysis of FOXM1 expression in indicated HCT116 cells. f HUVECs were treated with the CM from HCT116 cells transfected with indicated miRNA mimics and vectors. Cell viability of HUVECs was measured by CCK8 assay. g HUVECs were co-cultured with HCT116 cells transfected with indicated miRNA mimics and vectors in transwell apparatus. Migrated HUVECs were quantified after co-culture for 24 h. Scale bar = 20 μm. h HUVECs were treated with the CM from indicated cells, and subjected to tube formation assay. Scale bar = 20 μm. *p < 0.05, **p < 0.01