Fig. 7.

FOXM1 inhibits miR-6868-5p transcription by promoter histone methylation via EZH2. a qRT-PCR analysis of miR-6868-5p expression in HCT116 cells transfected with control vector or FOXM1 expressing plasmid. b qRT-PCR analysis of pri-miR-6868 expression in HCT116 cells with FOXM1 overexpressing or knockdown. c qRT-PCR analysis of EZH2 expression in HCT116 cells with FOXM1 overexpressing or knockdown. d ChIP assay of the enrichment of FOXM1 on EZH2 promoter relative to IgG in HCT116 cells. A random region without FHREs served as a negative control (Neg). e HCT116 cells transfected with control vector or FOXM1-expressing plasmid were treated with GSK126 (5 μM). The pri-miR-6868 levels were examined by qRT-PCR after 48 h. f ChIP assay of the enrichment of H3K27me3 on miR-6868 promoter relative to IgG in indicated HCT116 cells. *p < 0.05, **p < 0.01