Table 3.
List of primers used in this study
| Primer name | Sequence of primer | Amplified gene/template |
|---|---|---|
| prHKN220F | 5’-AGGATCCTGGCAGTGCGCTGATAGCC-3′ | DNA PSA wildtype |
| prHKN220R | 5’-AGAATTCGCACAGCGCGCGCGATGAGC-3′ | DNA PSA wildtype |
| prABC1220F | 5’-AGGATCCGTGCAGGGCGCTTTCTTTG-3′ | DNA PSA wildtype |
| prABC1220R | 5’-AGAATTCCATTGGCGAGGCTTTGCTGTTC-3’ | DNA PSA wild type |
| prPEP220F | 5’-AGGATCCTTGCAGGTGGCGATTTGCGC-3’ | DNA PSA wild type |
| prPEP220R | 5’-AGAATTCAACCATGTGTGGAAGCGCCG-3’ | DNA PSA wild type |
| prTWT220F | 5’-AGGATCCAGCAGGCCTACGGACGC-3’ | DNA PSA wild type |
| prTWT220R | 5’-AGAATTTCCGACGCCCTGTTTGGC-3’ | DNA PSA wild type |
| Pr2CSysFW | 5’-AGGATCCGGATGGTGCCGGAAGC-3’ | DNA PSA wild type |
| Pr2CSysRW | 5’-AGAATTCTTTGCGGGAACCAGAGCGG-3’ | DNA PSA wild type |
| prASP220F | 5’-AGGATCCGCGCTGTACGCGCTCG-3’ | DNA PSA wild type |
| prASP220R | 5’-AGAATTTGCTCAGAACCGCAATCCGCA | DNA PSA wild type |