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. 2018 Nov 5;128(12):5428–5433. doi: 10.1172/JCI121935

Figure 2. CS exposure and SHIV infection synergistically induce mucous cell hyperplasia and MUC5AC mucin expression.

Figure 2

(A) Quantification of secretory mucin MUC5AC and MUC5B mRNA levels in each group. RNA was isolated from frozen lung tissues and assessed using real-time qRT-PCR. (B) Micrographs of small airways showing MUC5AC (red) and HIV-gp120 (green) immunopositivity from each group, and the percentage of MUC5AC+ epithelial cells. Scale bars: 5 μm. (C) Micrographs of small airways showing MUC5B (green) immunopositivity from each group, and the percentage of MUC5B+ epithelial cells. Scale bars: 5 μm. (D) Representative micrographs of small airways showing mucopolysaccharides stained with AB-PAS. Scale bars: 5 μm. AB-PAS+ cells were quantified per unit length (mm) of basal lamina (BL). Data are mean ± SEM, n = 4–7/group, 1-way ANOVA; *P ≤ 0.05; **P < 0.01; ***P < 0.001.