Chamaejasmine intake by gavage inhibits HEp-2 tumor growth in athymic nude mice via inactivation of Akt/PKB-signaling pathways. Chamaejasmine was provided to the animals two weeks after cell inoculation. Chamaejasmine was provided orally with 0.5% methyl cellulose and 0.025% Tween 20 as vehicle to these animals on a daily basis. Group I, control, received 0.2 mL vehicle only, Group II received 10 mg/kg weight chamaejasmine and Group III received 30 mg/kg weight chamaejasmine daily for eight weeks. (A) (B) Tumor volume (mm3) in control and treated groups. (C) Quantitative measurement of apoptosis as demonstrated by M30 reactivity for HEp-2 tumors after chamaejasmine intake at the indicated doses. * p < 0.05. (D) Immunoblots for Akt, phospho-Ser(473)-Akt, GSK-3β, p-GSK-3β, BAD, p-BAD-Ser136, pro and cleaved caspase-9, pro and cleaved caspase-3 in tumor lysates after chamaejasmine intake at the indicated doses. The blots were stripped and reprobed with anti-β-actin antibody to ensure equal protein loading.