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Buffers for chromatographic determination of amino acids.
| Buffer Number | ||||
|---|---|---|---|---|
| Compound (g per 1 L) | 1 | 2 | 3 | 4 |
| Citric acid | 11.1 | 10.0 | 7.5 | – |
| Trisodium citrate | 4.0 | 5.6 | 9.1 | 19.6 |
| Sodium chloride | 9.3 | 8.4 | 18.0 | 52.6 |
| Boric acid | – | – | – | 2.1 |
| Thidiglycol | 2.5 | 2.5 | 2.5 | – |
| Sodium hydroxide | – | – | – | 0.5–3.0 |
| pH | 2.7 | 3.0 | 4.3 | 7.9 |
