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Apparent kinetic parameters of NahKs.
| Enzymes | NahK1_ATCC15697 | NahK_ATCC55813 | ||||
|---|---|---|---|---|---|---|
| Substrate | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) |
| ATP a | 0.10 ± 0.03 | 1.1 ± 0.1 | 11.0 | 0.11 ± 0.03 | 1.3 ± 0.1 | 11.8 |
| GlcNAc | 0.06 ± 0.01 | 0.95 ± 0.01 | 15.8 | 0.06 ± 0.01 | 1.1 ± 0.1 | 18.3 |
| ATP b | 0.08 ± 0.03 | 0.38 ± 0.02 | 4.8 | 0.06 ± 0.02 | 0.48 ± 0.03 | 8.0 |
| GalNAc | 0.09 ± 0.05 | 0.46 ± 0.07 | 5.1 | 0.08 ± 0.03 | 0.57 ± 0.04 | 7.1 |
a The other substrate is GlcNAc; b The other substrate is GalNAc.
