Table 2.
Substrate specificity of NahKs using GlcNAc, GalNAc, and their derivatives.
| Substrates | Percentage Conversion (%) | Substrates | Percentage Conversion (%) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| NahK_ATCC15697 | NahK_ATCC55813 | NahK_ATCC15697 | NahK_ATCC55813 | |||||||
| a 0.75 μM | b 15 μM | a 0.75 μM | b 15 μM | a 0.75 μM | b 15 μM | a 0.75 μM | b 15 μM | |||
1 GlcNAc |
35.4 ± 0.1 | NA | 42.3 ± 0.2 | NA |
11 GalNAc |
12.5 ± 0.1 | NA | 19.9 ± 0.1 | NA | |
2 GlcNTFA |
10.7 ± 0.9 | NA | 16.2 ± 0.9 | NA |
12 GalNTFA |
11.2 ± 1.6 | NA | 21.8 ± 0.2 | NA | |
3 GlcNAcN3
|
11.5 ± 1.0 | NA | 22.8 ± 0.4 | NA |
13 GalNAcN3
|
9.9 ± 0.6 | NA | 21.0 ± 1.2 | NA | |
4 GlcNBu |
20.9 ± 0.6 | NA | 35.0 ± 2.0 | NA |
14 GalNBu |
12.1 ± 0.3 | NA | 24.0 ± 0.1 | NA | |
5 GlcNBz |
10.3 ± 0.4 | NA | 5.2 ± 0.2 | NA |
15 GalNBz |
0 | 62.2 ± 1.0 | 0 | 51.9 ± 0.5 | |
6 GlcN3
|
0 | 14.5 ± 0.1 | 0 | 7.0 ± 0.1 |
16 GalN3
|
0 | 7.6 ± 0.1 | 0 | 4.3 ± 0.1 | |
7 GlcNH2
|
0 | 15.0 ± 0.1 | 0 | 8.4 ± 0.1 |
17 GlcNAc6S |
0 | 11.7 ± 0.2 | 0 | 6.6 ± 0.1 | |
8 GlcNS |
0 | 6.4 ± 0.2 | 0 | 4.0 ± 0.1 |
18 GlcNTFA6S |
0 | 7.2 ± 0.1 | 0 | 3.3 ± 0.2 | |
9 GlcNAc6Me |
4.4 ± 1.2 | 41.8 ± 0.3 | 2.1 ± 0.2 | 36.3 ± 0.3 |
19 GlcN36S |
0 | 6.9 ± 0.1 | 0 | 4.4 ± 0.1 | |
10 GlcNAc6N3
|
0 | 37.2 ± 0.5 | 0 | 23.4 ± 0.1 |
20 GlcN33S |
0 | 4.9 ± 0.1 | 0 | 3.9 ± 0.1 | |
NA: not assayed; a Reactions were allowed to proceed for 10 min at 37 °C; b Reactions were allowed to proceed for 30 min at 37 °C.