Table 3.
Substrate specificity of NahKs using Glc, Gal, Man, ManNAc, and their derivatives.
| Substrates | Percentage Conversion (%) | Substrates | Percentage Conversion (%) | ||
|---|---|---|---|---|---|
| NahK_ATCC15697 | NahK_ATCC55813 | NahK_ATCC15697 | NahK_ATCC55813 | ||
21 Glc |
9.1 ± 0.1 | 4.7 ± 0.1 |
28 Gal |
7.3 ± 0.2 | 4.4 ± 0.1 |
22 2-deoxyGlc |
44.8 ± 0.2 | 28.4 ± 0.1 |
29 ManNAc |
8.9 ± 0.1 | 5.5 ± 0.1 |
23 Man |
68.0 ± 1.7 | 37.1 ± 0.4 |
30 ManNGc |
7.6 ± 0.1 | 5.4 ± 0.2 |
24 2F-Man |
44.4 ± 0.2 | 47.0 ± 0.1 |
31 ManNAcF |
12.0 ± 0.1 | 9.1 ± 0.2 |
25 2Me-Man |
9.4 ± 0.5 | 0 |
32 ManNAcOMe |
12.0 ± 0.4 | 7.4 ± 0.3 |
26 2N3-Man |
53.3 ± 0.1 | 40.2 ± 0.2 |
33 ManNAcN3
|
20.3 ± 0.3 | 18.6 ± 0.4 |
27 4-deoxyMan |
37.1 ± 0.2 | 23.9 ± 0.1 |
34 ManNAc6OMe |
32.6 ± 0.1 | 28.9 ± 0.1 |
The concentration of the enzyme used was 15 μM. Reactions were allowed to proceed for 30 min at 37 °C.